Many pathologies of skin, especially ageing and cancer, involve modifications in the matrix alignment. Such tissue reorganization\ncould have impact on cell behaviour and/ormore global biological processes. Tissue engineering provides accurate study model by\nmimicking the skin and it allows the construction of versatile tridimensional models using human cells. It also avoids the use of\nanimals, which gave sometimes non translatable results. Among the various techniques existing, the self-assembly method allows\nproduction of a near native skin, free of exogenous material. After cultivating human dermal fibroblasts in the presence of ascorbate\nduring two weeks, a reseeding of these cells takes place after elevation of the resulting stroma on a permeable ring and culture\npursued for another two weeks. This protocol induces a clear realignment of matrix fibres and cells parallel to the horizon. The\nthickness of this stretched reconstructed tissue is reduced compared to the stroma produced by the standard technique. Cell count\nis also reduced. In conclusion, a new, easy, and inexpensive method to produce aligned tissue free of exogenous material could be\nused for fundamental research applications in dermatology.
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